桦褐孔菌菌丝体及甾类化合物的发酵条件优化
中文摘要: 对桦褐孔菌深层发酵培养基进行了筛选，以菌丝体及甾类化合物产量为目标对发酵条件进行了优化，确定最佳发酵条件为：30g/L葡萄糖，2.5g/L黄豆粉，2.5g/L蛋白胨，3g/L KH2PO4，0.8g/L MgSO4，0.8g/L CaSO4，初始pH4.0，接种量15%，装液量100mL/500mL，转速150r/min，28℃恒温培养。此条件下培养11d，菌丝体干重达12.52g/L，甾体类化合物的产量达112.44mg/L。英文摘要: To produce mycelial biomass and steroids, the optimization of submerged fermentation condition for Inonotus obliquus was carried out. The optimum fermentation medium was as follows: 30g/L glucose, 2.5g/L Soybean powder, 2.5g/L peptone, 3g/L KH2PO4, 0.8g/L MgSO4, 0.8g/L CaSO4. The initial pH was 4.0, and the inoculation volume was 15%. The fungus was cultured at 28℃ in 500mL shake flasks containing 100mL of medium at 150r/min. Under this condition, mycelial biomass of 12.52g/L was obtained while production of steroids about 112.44mg/L.黄伟,陆震鸣,耿燕,许泓瑜,史劲松,许正宏.桦褐孔菌菌丝体及甾类化合物的发酵条件优化
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