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桦褐孔菌菌丝体及甾类化合物的发酵条件优化

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发表于 2014-7-10 20:01:19 | 显示全部楼层 |阅读模式
中文摘要:      对桦褐孔菌深层发酵培养基进行了筛选,以菌丝体及甾类化合物产量为目标对发酵条件进行了优化,确定最佳发酵条件为:30g/L葡萄糖,2.5g/L黄豆粉,2.5g/L蛋白胨,3g/L KH2PO4,0.8g/L MgSO4,0.8g/L CaSO4,初始pH4.0,接种量15%,装液量100mL/500mL,转速150r/min,28℃恒温培养。此条件下培养11d,菌丝体干重达12.52g/L,甾体类化合物的产量达112.44mg/L。
  英文摘要:      To produce mycelial biomass and steroids, the optimization of submerged fermentation condition for Inonotus obliquus was carried out. The optimum fermentation medium was as follows: 30g/L glucose, 2.5g/L Soybean powder, 2.5g/L peptone, 3g/L KH2PO4, 0.8g/L MgSO4, 0.8g/L CaSO4. The initial pH was 4.0, and the inoculation volume was 15%. The fungus was cultured at 28℃ in 500mL shake flasks containing 100mL of medium at 150r/min. Under this condition, mycelial biomass of 12.52g/L was obtained while production of steroids about 112.44mg/L.黄伟,陆震鸣,耿燕,许泓瑜,史劲松,许正宏.桦褐孔菌菌丝体及甾类化合物的发酵条件优化 桦褐孔菌菌丝体及甾类化合物的发酵条件优化.pdf (575.85 KB, 下载次数: 802)
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