Folin-Ciocalteu比色法测定桦褐孔菌多酚的条件优化
中文摘要: 以没食子酸为标准品，通过正交试验和单因素实验研究了Folin-Ciocalteu 比色法测定桦褐孔菌中多酚含量的适宜条件。结果表明，优化后的显色条件为Folin-Ciocalteu试剂用量0.3mL、10% Na2CO3溶液0.6mL，25℃时避光反应30min，于750nm处测定其吸光值。多酚质量浓度在0.0032－0.0256mg/mL范围内与吸光值有良好的线性关系。根据拟合的线性回归方程对桦褐孔菌多酚进行定量测定，野生桦褐孔菌和人工培养桦褐孔菌中多酚含量最高分别为（72.05±0.08）mg/g、（52.76±0.06）mg/g（n=6）。不同加标水平的加标回收率测定实验获得的平均回收率为98.95%，RSD为1.27%。该法测定桦褐孔菌多酚具有快速、准确，稳定性强，重现性好，精密度高的特点，可应用于实际样品的测定。英文摘要: A quantitative method for the determination of polyphenols in sclerotia or mycelia of Inonotus obliquus was studied by orthogonal and single factor experiment with Folin-Ciocalteu reagent and standard of Gallic acid. The results showed that the content of polyphenols in I. obliquus can be well calculated according to their colorimetric absorption at 750nm by applying Folin-Ciocalteu reagent 0.3mL and 10% Na2CO3 0.6mL at 25℃ for 30min. The linear range of calibration curve was 0.0032－0.0256mg/mL for the gallic acid (r2=0.9975). Using the optimezed method, the content of polyphenols in sclerotia was determinated to be (72.05±0.08)mg/g and mycelia to be (52.76±0.06)mg/g (n=6). The average recovery was 98.95% (RSD=1.27%). Therefore, the optimized method can dectect the content of polyphenols in I. obliquus in a swift, accurate and practical way with high reproducibility.
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