纳米级灵芝子实体粉末和破壁灵芝孢子粉体外抗肿瘤活性研究

春雨菌业

中文摘要:

对纳米级灵芝子实体粉末及破壁灵芝孢子粉石油醚提取物（PE）、氯仿提取物（CE）、丙酮（AE）、甲醇提取物（ME）、水提取物（WE）与灵芝子实体及灵芝孢子提取量进行对比，利用GC-MS联用仪对石油醚提取物进行了成分分析鉴定，对水提物中总糖进行了含量测定，并利用宫颈癌细胞Hela和晶体上皮细胞SRA01/04进行了体外增殖作用和剂量效应关系研究，为灵芝资源的保护及进一步开发利用提供理论基础。结果表明，纳米化灵芝子实体及破壁灵芝孢子不同溶剂提取量显著增加，纳米级灵芝子实体粉末水提取物具有抑制宫颈癌细胞Hela和晶体上皮细胞SRA01/04增殖的作用。破壁灵芝孢子各溶剂提取物对宫颈癌细胞Hela和晶体上皮细胞SRA01/04没有明显的增殖抑制作用。

　The yields of petroleum ether extract (PE)， chloroform extract (CE)， acetone extract (AE)， methanol extract (ME) and water extract (WE) of Ganoderma lucidum nano- and micron-sized fruiting body powder， and the yields of PE， CE， AE， ME as well as WE of Ganoderma lucidum wall-broken nano-sized and wall-unbroken spore powder， were compared. GC-MS spectrometer was used for analysis and identification of constituents of the PEs. The total sugars of the WEs were determined. In vitro proliferation effect on the human uterine cervical cancer cell line (Hela cells) and the human lens epithelial cells line (SRA01/04 cells) and dose effect relationship of the aqueous extracts were studied. This study aims at providing a theoretical basis for the resource protection and further development and utilization of Ganoderma lucidum. The results showed that the different solvent extraction yields of the nano-sized Ganoderma lucidum fruiting body powder and the wall-broken nano-sized spore powder increased significantly. The aqueous extract of the nano-sized Ganoderma lucidum fruiting body powder has an inhibitory effect on the proliferation of Hela cells and SRA01/04 cells， while the various solvent extracts of the wall-broken nano-sized spore powder did not show obvious proliferation inhibition.

纳米级灵芝子实体粉末和破壁灵芝孢子粉体外抗肿瘤活性研究.pdf (171.63 KB, 下载次数: 224)

2014-6-11 20:33 上传

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